Protein Analysis of Fruit Fly After Exposure to a 5 Tesla Static Magnetic Field


Tamami KAWASAKI, Takao KOANA
We studied whether static magnetic fields have an effect on biological systems at protein level, using two-dimensional polyacrylamide gel electrophoresis(2D-PAGE). After 30 min exposure of male fruit flies to a 5 T static magnetic field, one protein spot on 2D gel disappeared and a new spot appeared near the former one. But this protein pattern change was not found after prolonged exposure (6h). In addition the change was not detected after heat shock treatment and there should be a different mechanism to cause change from expression of heat shock genes. As this protein was solely detected in central nervous system, the phenomenon reflects adaptation of flies to the magnetic field exposure at the molecular level.